SHENTEK® Residual E. coli RNA Quantitation Kit (2G) is used for quantitation of host cell RNA from E. coli in a variety of biopharmaceutical products. This kit employs duplex reverse transcription quantitative PCR technique to performs a rapid, specific, and reliable quantitative detection of residual E. coli RNA. The target gene (FAM) can be determined at the femtogram (fg) level, whereas IPC-Internal Positive Control (VIC) is also included in the E. coli RNA Primer&Probe MIX to evaluate the performance of each PCR reaction.
| Components | Shipping Condition | Unit Size | Detection Method |
| E.coli RNA Control | -20℃ | 100 Reactions | Probe-based qPCR |
| One Step qPCR Buffer | -20℃, protect from light | ||
| One Step Enzyme MIX | -20℃, protect from light | ||
| E.coli RNA Primer&Probe MIX (Incl IPC) | -20℃, protect from light | ||
| RNase-Free H2O | -20℃ |
| Range | 2x10-3-2x102pg/μL, R2 =1.000，E=104.6% |
| Accuracy | 81.1%-107.0%, CV<30% |
| LOQ | 0.002 pg/μL |
| Precision | 7.4%-11.0% (<30%) |
| Robustness | Freeze-thaw stability over 5 cycles |
| Instrument suitability not limited to Thermo, Biorad, Bioer, SHENTEK qPCR equipment |
SHENTEK® Residual Host Cell RNA Sample Preparation Kit
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