The ResHCDTM Residual Host Cell DNA Quantitation Kit series provides a comprehensive and reliable solution for monitoring residual host cell DNA levels in biopharmaceutical products and drug substance samples derived from the engineered production cell lines.
The ResHCDTM Plasmid DNA Quantitation Kit is specifically designed for highly sensitive quantitation of residual Plasmid genomic DNA. By integrating optimized primer design with advanced qPCR reagent chemistry, this kit enables rapid, highly specific, and reliable detection of trace levels of residual Plasmid DNA with femtogram-level sensitivity.
The product described herein is for Research Use Only and is not intended for diagnostic or therapeutic use.
| Components | Shipping Condition | Unit Size | Detection Method |
| qPCR Reaction MIX-1 | -20℃, protect from light | 100 Reactions | Probe-based qPCR |
| Plasmid Primer&Probe MIX | |||
| IPC MIX-6 | |||
| DNA Dilution Buffer (DDB) | -20℃ | ||
| Plasmid linear DNA Control |
Wide Dynamic Range for Accurate Quantitation
High-quality DNA standards enable robust standard curve generation with excellent linearity across a broad dynamic range of 4.97 pg/μL to 497 ng/μL, supporting accurate quantitation of residual Plasmid DNA from complex process intermediates to highly purified final biopharmaceutical products.
Superior Specificity for Plasmid Genomic DNA
Optimized primer design provides exceptional specificity toward Plasmid genomic DNA with no detectable cross-reactivity, ensuring reliable and accurate residual DNA quantitation across diverse sample matrices.
Streamlined Workflow with High Reproducibility
A simplified workflow integrating sample preparation, qPCR amplification, and data analysis enables rapid, consistent, and reproducible results. When combined with the SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Fast), the system delivers efficient DNA recovery and reliable quantitation even from complex biological matrices.
Enhanced Stability for Global Distribution
Optimized enzyme formulations and proprietary stabilizing components enhance reagent robustness against temperature fluctuations, enabling reliable transportation and extended storage stability. The assay maintains consistent performance after storage at 2–8°C for up to two months, with no significant impact on assay performance observed.
| Linearity and range | 4.97x101 to 4.97x105 pg/μL, R2=0.99986, E=96.0% |
| Accuracy | Recovery range: 95.4% to 110.8%, CV values: <15 % |
| Quantitation limit (QL) | 5.11 copies/μL |
| precision | CV values: 4.8% to 12.5% |
| Specificity | No detectable cross-reactivity amount HEK293 genome |
| Repeatability | Sample with three concentration level, CV values: 7.5% to 9.9% |
| Robustness | Instrument suitability not limited to Thermo, Biorad, SHENTEK qPCR equipments |
| Freeze-thaw stability over 5 cycles |
SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Fast)
Email: info@shentekbio.com
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info@shentekbio.com 
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