The SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Fast) is specifically designed for rapid and efficient pretreatment of residual host cell DNA (HCD) samples from biological products and process intermediates.
Leveraging advanced magnetic particle technology combined with an optimized lysis system, the kit enables high-efficiency isolation and purification of trace levels of residual host cell DNA across a broad range of sample matrices, including challenging samples containing potential interfering substances such as chondroitin sulfate and heparin. The resulting purified DNA samples are optimized for downstream quantitative residual DNA analysis, ensuring reliable, consistent, and reproducible performance.
This kit provides flexible extraction options to accommodate different laboratory workflows and automation requirements:
1. rHCDpurify® System
Combined with the rHCDpurify® system, the kit provides a fully automated sample pretreatment workflow, enabling streamlined sample lysis and nucleic acid extraction through a simple one-click operation.
2. Thermo Scientific™ KingFisher™ Flex System
The kit is validated for use with the Thermo Scientific™ KingFisher™ Flex platform. An optimized protocol file (ResDNA Sample Preparation Kit (Fast) for KingFisher Flex) is available for download and can be imported directly into the instrument, enabling a streamlined one-click automated extraction workflow.
3. Customized Extraction Workflow Development
The kit can be further adapted for customer-developed extraction platforms or customized automated workflows, providing flexible integration options to meet specific laboratory requirements and workflow configurations.
The kit is for Research Use Only and is not for use in diagnostic procedures or for therapeutic purposes in humans or animals.
| Components | Shipping Condition | Unit Size | Detection Method |
| Wash buffer A | Room Temperature | 100 Reactions | Magnetic particle separation |
| Lysis buffer I | |||
| Lysis buffer Ⅱ | |||
| Elution buffer | |||
| Dilution buffer | |||
| Magnetic particles | 2-8℃ | ||
| Proteinase K | -20℃ |
| Recovery | > 80% |
| Repeatability | CV values < 30% |
The optimized processing strategy significantly shortens extraction time (~30-45 minutes) while maintaining high extraction efficiency, offering an optimal balance between speed and performance.
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