Residual BHK DNA Size Analysis Kit is used to quantitate BHK DNA residues of different fragment sizes in different stages of biopharmaceutical products, from in-process samples to final products.
SHENTEK® Residual BHK DNA Size Analysis Kit is used to quantitate BHK DNA residues of different fragment sizes in different stages of biopharmaceutical products, from in-process samples to final products. This kit utilizes quantitative PCR (FAM) technique to perform rapid and specific quantitation of residual BHK DNA fragments in samples. It is designed to amplify four different fragments (81bp, 134bp, 216bp, 589bp) for the accurate size distribution, and at the limit of detection (LOD) reaches femtograms (fg) level. For extraction information, please refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191).
| Components | Shipping Condition | Unit Size | Detection Method |
| BHK DNA Control | -20℃ | 4×100 Reactions | Probe-based qPCR |
| qPCR Reaction Buffer | -20℃, protect from light | ||
| BHK Primer&Probe MIX-81 | |||
| BHK Primer&Probe MIX-134 | |||
| BHK Primer&Probe MIX-216 | |||
| BHK Primer&Probe MIX-589 | |||
| IPC MIX | |||
| DNA Dilution Buffer (DDB) | -20℃ |
| Range | St1~St5：0.03~300 pg/μL（3 ng/rxn~300 fg/rxn）R2>0.990，83.3%≤E≤110.0% | ||
| LOQ | 0.03 pg/μL（300 fg/rxn） | ||
| Robustness | Freeze-thaw stability over 4 cycles | ||
| Instrument suitability not limited to Thermo, SHENTEK qPCR equipments | |||
SHENTEK® Residual Host Cell DNA Sample Preparation Kit
SHENTEK® Residual BHK DNA Quantitation Kit
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