MVM Detection Kit is a real-time qPCR test kit for rapid detection of Minute Virus of Mice (MVM) contamination introduced by murine-derived cells (CHO, BHK, NS0, etc.), animal-derived raw materials and laboratory environments in biological products.
SHENTEK® MVM Detection Kit is designed for qualitative detection of Minute Virus of Mice (covering i, p, m and c strains) contamination in mouse-derived cell lines used in biopharmaceutical production processes, for example CHO, BHK, and NS0 cells. The kit works together with SHENTEK® Virus DNA & RNA Extraction Kit (Product No.1506730).
The detection assay is capable of detecting four MVM strains (i, p, m and c), and could reach a sensitivity of 50 copies per reaction, meanwhile shows high specificity without cross-amplification with engineered cell lines (CHO, VERO, 293T, MDCK, NS0, and Sf9) or engineered bacteria genomes (E. coli, Pichia pastoris). MVM Positive Control and VIR Internal Control (IC) are provided to ensure reliable results. Combined with SHENTEK® Virus DNA & RNA Extraction Kit, this kit can efficiently recover and detect MVM DNA from complex sample matrices, such as cells and serum.
| Components | Shipping Condition | Unit Size | Product Line | Detection Method |
| VIR Internal Control (IC) | '-20℃ | 50 Reactions | SHENTEK® | Probe-based qPCR |
| DNA Dilution Buffer (DDB) | ||||
| qPCR Master MIX | '-20℃, protect from light | |||
| MVM Primer&Probe MIX | ||||
| VIR IPC MIX |
| LOD | 1 copy/μL (plasmid DNA) |
| Specificity | No cross-reactivity with CHO, Vero, 293T, MDCK, Sf9, NS0, MDBK, PK15, E. coli and Pichia pastoris cells |
| Robustness | Instrument suitability not limited to SHENTEK-96S, Thermo ABI 7500, and Roche LightCycler 480 Ⅱ |
SHENTEK® Virus DNA & RNA Extraction Kit
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