ResHCDTM Vero DNA Quantitation Kit

Name: ResHCDTM Vero DNA Quantitation Kit
Brand: SHENTEKBIO
Availability: InStock

For Research Use Only (RUO)

Product Number: 1101121

The ResHCD^TM Residual Host Cell DNA Quantitation Kit series provides a comprehensive and reliable solution for monitoring residual host cell DNA levels in biopharmaceutical products and drug substance samples derived from the engineered production cell lines.

The ResHCD^TM Vero DNA Quantitation Kit is specifically designed for highly sensitive quantitation of residual Vero genomic DNA. By integrating optimized primer design with advanced qPCR reagent chemistry, this kit enables rapid, highly specific, and reliable detection of trace levels of residual Vero DNA with femtogram-level sensitivity.

The product described herein is for Research Use Only and is not intended for diagnostic or therapeutic use.

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Overview- ResHCDTM Vero DNA Quantitation Kit

Components	Shipping Condition	Unit Size	Detection Method
qPCR Reaction MIX-2	-20℃, protect from light	100 Reactions	Probe-based qPCR
Vero Primer&Probe MIX
IPC MIX-2
DNA Dilution Buffer (DDB)	-20℃,
Vero DNA Control

Key Features -SHENTEK^® ResHCD^TM Vero DNA Quantitation Kit

Wide Dynamic Range for Accurate Quantitation

High-quality DNA standards enable robust standard curve generation with excellent linearity across a broad dynamic range of 1 fg/μL to 300 pg/μL, supporting accurate quantitation of residual Vero cell DNA from complex process intermediates to highly purified final biopharmaceutical products.

Superior Specificity for Vero cell Genomic DNA

Optimized primer design provides exceptional specificity toward Vero cell genomic DNA with no detectable cross-reactivity, ensuring reliable and accurate residual DNA quantitation across diverse sample matrices.

Streamlined Workflow with High Reproducibility

A simplified workflow integrating sample preparation, qPCR amplification, and data analysis enables rapid, consistent, and reproducible results. When combined with the SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Fast), the system delivers efficient DNA recovery and reliable quantitation even from complex biological matrices.

Enhanced Stability for Global Distribution

Optimized enzyme formulations and proprietary stabilizing components enhance reagent robustness against temperature fluctuations, enabling reliable transportation and extended storage stability. The assay maintains consistent performance after storage at 2–8°C for up to two months, with no significant impact on assay performance observed.

Specification -SHENTEK^® ResHCD^TM Vero DNA Quantitation Kit

Linearity and range	1.00x10-3 to 3.00x102 pg/μL, R2=0.99894, E=96.1%
Accuracy	Recovery range: 78.0% to 88.3%, CV values: < 20%
Quantitation limit (QL)	0.00091 pg/μL
Detection limit (DL)	0.0005 pg/μL
Precision	CV values: 2.4% to 17.9%
Specificity	No detectable cross-reactivity with CHO, E. coli and Pichia Pastoris.
Repeatability	Sample with three concentration level, CV values: 2.6% to 10.0%
Robustness	Instrument suitability not limited to Thermo, Biorad, SHENTEK qPCR equipments
Robustness	Freeze-thaw stability over 5 cycles