Bovine Virus Detection Kit is a real-time RT-qPCR test kit for rapid detection of Bovine Viral Diarrhea Virus (BVDV), Reovirus 3 (REO-3), Bovine Parainfluenza 3 Virus (PI-3), Bovine Parvovirus type 3 (BPV-3), and Bovine Adenovirus type 3 (BAV-3).
SHENTEK® Bovine Virus Detection Kit (BVDV/REO-3/PI-3/BPV-3/BAV-3) is designed for rapid and qualitative detection of five bovine-derived viruses contaminants in serum and cell bank, including Bovine Viral Diarrhea Virus (BVDV), Reovirus 3 (REO-3), Bovine Parainfluenza 3 Virus (PI-3), Bovine Parvovirus 3 (BPV-3), and Bovine Adenovirus 3 (BAV-3). The kit works together with SHENTEK® Virus DNA & RNA Extraction Kit (Product No.1506730).
Using multiplex real-time PCR technique, the assay can simultaneously detect three RNA viruses (BVDV/REO-3/PI-3) or two DNA viruses (BPV-3/BAV-3) in a single well, allowing for rapid and efficient detection of bovine-derived viral in raw materials of animal origin, cell banks, virus seed banks, unprocessed bulk, and final products in bioproducts process. The detection assay shows high sensitivity and specificity with a detection limit of 50 copies per reaction. Positive Control (can be purchased separately) and Internal Control (IC) are provided to ensure reliable results. Combined with SHENTEK® Virus DNA & RNA Extraction Kit, these kits can efficiently recover and detect bovine virus nucleic acid from complex sample matrices such as cells and serum.
| Components | Shipping Condition | Unit Size | Product Line | Detection Method |
| 5×Multiplex RT-PCR Buffer | '-20℃ | 50 Reactions | SHENTEK® | Probe-based qPCR |
| Multiplex RT-PCR Enzyme MIX | ||||
| Multiplex qPCR Reaction Buffer | ||||
| VIR Internal Control (IC) | ||||
| RNase-Free H2O | ||||
| BV RNA Primer&Probe MIX | '-20℃, protect from light | |||
| BV DNA Primer&Probe MIX | ||||
| Multiplex IPC MIX |
| LOD | 1 copy/μL (plasmid DNA) |
| Specificity | No cross-reactivity with CHO, Vero, 293T, MDCK, Sf9, NS0, MDBK, PK15, E. coli and Pichia pastoris cells. |
| Robustness | Instrument suitability not limited to SHENTEK-96S, Thermo ABI 7500, and Roche LightCycler 480 Ⅱ |
SHENTEK® Virus DNA & RNA Extraction Kit
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