Fungi DNA Detection Kit can qualitatively detect the presence of fungal DNA in samples, covering approximately 92% of known fungal species and matching nearly 5,000 fungal DNA sequences (or subspecies).
MicroSHENTEK® Fungi DNA Detection Kit is used together with MicroSHENTEK® Fungi & Bacteria DNA Extraction Kit, to qualitatively determine whether there is fungi contamination in cells, cell products or vaccines, etc. The assay performance is validated according to the Ch.P, EP, JP and USP qPCR method with a detection limit of not more than 35 CFU/reaction.
The detection kit uses real-time PCR technique to detect possible fungal DNA in the test samples, and is able to cover about 92% of known fungal species, which matches nearly 5,000 species (or subspecies) of fungal DNA sequences. The specificity and coverage has been validated in a variety of matrices, as well as on non-fungal species, production cells, and engineered bacteria.
For extraction information, please refer to the MicroSHENTEK® Fungi & Bacteria DNA Extraction Kit User Guide (Product No. 1504633).
| Components | Shipping Condition | Unit Size | Product Line | Detection Method |
| Fun qPCR Reaction Buffer | -20℃, protect from light | 50 Reactions | MicroSHENTEK | Probe-based qPCR |
| Fun Primer&Probe MIX | ||||
| Fun Internal Control (IC) | -20℃ | |||
| Fun Positive Control (PC) | ||||
| DNA Dilution Buffer (DDB) |
| LOD | 50-100 CFU |
| Specificity | No matrix interference with DMEM and RPMI1640 |
| No cross reactivity with 6 species of bacteria (Staphylococcus aureus, Pseudomonas aeruginosa, Clostridium sporogenes, Escherichia coli, Streptococcus pneumoniae, Bacillus subtilis), 3 species of mycoplasma (Mycoplasma orale, Mycoplasma pneumoniae, Mycoplasma hyorhinis), and 6 species of engineered cells (CHO, Vero, Sf9, 293T, HEK293, MDCK). | |
| Detect about 92% of known fungal species | |
| Robustness | Freeze-thaw stability over 3 cycles |
| Instrument suitability not limited to SHENTEK-96S and LightCycler 480 Ⅱ |
MycoSHENTEK® Fungi & Bacteria DNA Extraction Kit
MycoSHENTEK® Bacteria DNA Detection Kit
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