Bacteria DNA Detection Kit can qualitatively detect the presence of bacterial DNA in samples, covering approximately 92% of known bacterial species and aligning with nearly 60,000 bacterial DNA sequences (or subspecies).
MicroSHENTEK® Bacteria DNA Detection Kit is used together with MicroSHENTEK® Fungi & Bacteria DNA Extraction Kit, to qualitatively determine whether there is bacteria contamination in cells, cell products or vaccines, etc. The assay performance is validated according to the Ch.P, EP, JP and USP qPCR method with a detection limit of not more than 35 CFU/reaction.
The detection kit uses real-time PCR technique to detect possible bacterial DNA in the test samples, and is able to cover about 92% of known bacterial species, which matches nearly 60,000 species (or subspecies) of bacterial DNA sequences. The specificity and coverage have been validated in a variety of matrices, as well as on non-bacteria species, production cells, and engineered fungi.
For extraction information, please refer to the MicroSHENTEK® Fungi & Bacteria DNA Extraction Kit User Guide (Product No. 1504633).
| Components | Shipping Condition | Unit Size | Product Line | Detection Method |
| Bac Positive Control (PC) | -20℃ | 50 Reactions | MicroSHENTEK | Probe-based qPCR |
| DNA Dilution Buffer (DDB) | ||||
| Bac qPCR Reaction Buffer | -20℃, protect from light | |||
| Bac Primer & Probe MIX |
| LOD | 50-100 CFU |
| Specificity | No matrix interference with DMEM and RPMI1640 |
| No cross reactivity with 2 species of fungi (Candida albicans, Aspergillus niger), 3 species of mycoplasma (Mycoplasma orale, Mycoplasma pneumoniae, Mycoplasma hyorhinis), and 6 species of engineered cells (CHO, Vero, Sf9, 293T, HEK293, MDCK) | |
| Detect about 92% of known bacteria species | |
| Robustness | Freeze-thaw stability over 3 cycles |
| Instrument suitability not limited to SHENTEK-96S and LightCycler 480 Ⅱ |
MycoSHENTEK® Fungi & Bacteria DNA Extraction Kit
MycoSHENTEK® Fungi DNA Detection Kit
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