Residual E. coli DNA Size Analysis Kit is used to quantitate residual E.coli DNA of different fragment sizes at various stages of biopharmaceutical products, from in-process samples to final products.
The SHENTEK® Residual E.coli DNA Size Analysis Kit (2G) utilizes real-time PCR technique to perform rapid and specific quantitation of residual E.coli DNA fragments (FAM) in samples. It is designed to amplify four different fragments (85bp, 103bp, 220bp and 550bp) for the accurate determination of their size distribution at the femtograms (fg) level.
For extraction information, please refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191).
Size: 4 × 100 Reactions
| Components | Shipping Condition | Unit Size | Detection Method |
| E.coli DNA Control | -20℃ | 4×100 Reactions | Probe-based qPCR |
| qPCR Reaction Buffer | -20℃, protect from light | ||
| E.coli Primer&Probe MIX-85 | |||
| E.coli Primer&Probe MIX-103 | |||
| E.coli Primer&Probe MIX-220 | |||
| E.coli Primer&Probe MIX-550 | |||
| IPC MIX | |||
| DNA Dilution Buffer (DDB) | -20℃ |
| Range | 85bp, 103bp, 220bp and 550bp: 3x10-2-3x102 pg/μL, R2 ≥0.990, E= 83.3%-110% | ||
| Accuracy | Recovery rate = 91.6%-102.5% | ||
| LOQ | 85bp, 134bp, 229bp, 552bp: 0.03 pg/μL | ||
| Precision | 3.2%-22.1% ( ≤40%) | ||
| Robustness | Batch-to-batch precision (CV = 13.9%-30.6%) | ||
SHENTEK® Residual Host Cell DNA Sample Preparation Kit
SHENTEK® Residual E. coli DNA Quantitation Kit
SHENTEK® Residual E. coli DNA Quantitation Kit (2G)
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SHENTEK® E.coli (Protein Expression Strains) HCP ELISA Kit (One-step ELISA)
SHENTEK® E.coli (K-12 & Alkaline Lysis) HCP ELISA Kit
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