SHENTEK® Residual E. coli DNA Quantitation Kit is used for quantitation of residual E. coli host cell DNA in a variety of biopharmaceutical products. This kit uses quantitative PCR to perform rapid and specific quantitation of residual DNA at the femtogram level. The assay is accurate and reliable across a broad range of sample types, from in-process samples to final products. The kit comes with E. coli Control DNA, which is traceable to a primary DNA reference standard.
For extraction information, please refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. SK030203D100).
| Components | Shipping Condition | Unit Size | Detection Method |
| E. coli DNA Control | -20℃ | 100 Reactions | Probe-based qPCR |
| E. coli qPCR MIX | -20℃, protect from light | ||
| DNA Dilution Buffer (DDB) | -20℃ |
| Range | 3x10-2-3x102 pg/μL, R2=0.99968, E=97.5% |
| Accuracy | Recovery = 81.2%-107.4% , CV<30% |
| LOQ | 0.03 pg/μL |
| LOD | 0.003 pg/μL |
| Precision | 2.8%-17.9% (<30%) |
| Robustness | Freeze-thaw stability over 5 cycles |
| Instrument suitability not limited to Thermo, Biorad, SHENTEK qPCR equipments |
SHENTEK® Residual Host Cell DNA Sample Preparation Kit
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SHENTEK® E.coli (Protein Expression Strains) HCP ELISA Kit (One-step ELISA)
SHENTEK® E.coli (K-12 & Alkaline Lysis) HCP ELISA Kit
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