Lentivirus vector are used to introduce CAR or TCR genetic sequence into cell therapy products. However, due to the variations among different individuals' genomes, there maybe variable copy numbers of the lentivirus-specific TCR gene transferred into peripheral T cells, resulting in different cyto-toxicity in heterogeneous CAR-T cell populations.
SHENTEK CAR/TCR Gene Copy Number Quantification Kit is are liable and accurate quality control test for the detection of transgene copy number in CAR-T cells that are transfected with rHIV-1 lentiviral vectors.
Range: CAR/TCR: 2.33×101-2.33×107 copies/μL, R2= 0.999829,E=94.9%
SCG: 2.33×102-2.33×107, R2= 0.999564,E=94.8%
LOQ: CAR/TCR: 2.33×101 copies/μL
SCG: 5.285×101 copies/μL
LOD: 2.33 copies/μL
Precision: 5.9%-9.1% (CV<15%)
Email: info@shentekbio.com
Telephone:
US Mobile: +1-908-822-3199
US Office: +1-800-960-3004 ext 720
China Office: +86-400-878-2189
Address:
USA site: 3350 Scott Blvd Bldg 6, Santa Clara, CA 95054
China site: No.1366 Hongfeng Road, Huzhou, Zhejiang, China, 313000