Vesivirus 2117 Detection Kit is a real-time RT-qPCR test kit for rapid detection of Vesivirus 2117 contamination in mammalian cell lines (such as CHO cells) in biological products.
SHENTEK® Vesivirus 2117 Detection Kit is designed for qualitative detection of Vesivirus 2117 contamination in mammalian cell lines such as CHO cells used in biopharmaceutical production processes. The kit works together with SHENTEK® Virus DNA & RNA Extraction Kit (Product No.1506730).
The detection assay could reach a sensitivity of 50 copies per reaction, and shows high specificity without cross-amplification with bovine, porcine, and engineered cell lines (CHO, VERO, 293T, MDCK, NS0, and Sf9) or engineered bacteria (E. coli, Pichia pastoris) genomes. Vesivirus 2117 Positive Control and VIR Internal Control (IC) are provided to ensure reliable results. Combined with SHENTEK® Virus DNA & RNA Extraction Kit, these kits can efficiently recover and detect Vesivirus 2117 RNA from complex sample matrix such as cells and serum.
| Components | Shipping Condition | Unit Size | Detection Method |
| VIR Internal Control (IC) | -20℃ | 50 Reactions | Probe-based qPCR |
| 5×RT-qPCR Buffer | |||
| RNase-Free H2O | |||
| RT-qPCR Enzyme MIX | -20℃, protect from light | ||
| VV-2117 Primer&Probe MIX | |||
| VIR IPC MIX |
| LOD | 5 copies/μL (plasmid DNA) |
| Specificity | No cross-reactivity with CHO, Vero, 293T, MDCK, Sf9, NS0, MDBK, PK15, E. coli and Pichia pastoris cells |
| Robustness | Instrument suitability not limited to SHENTEK-96S, Thermo ABI 7500, and Roche LightCycler 480 Ⅱ |
SHENTEK® Virus DNA & RNA Extraction Kit
SHENTEK® Vesivirus 2117 Positive Control
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