The SHENTEK® Telomerase Assay kit can monitor the activity and growth status of stem cells and, to a certain extent, be used for in vitro characterization of the tumorigenicity of cells.
The SHENTEK® Telomerase Assay kit utilizes the Telomere Repeat Amplification Protocol (TRAP) and qPCR technology, featuring a dual-fluorescence qPCR system: assesses PCR inhibitors via an internal reference gene to prevent false negatives; precisely quantifies telomerase activity using TSR8 as a standard. It achieves high sensitivity and accurate quantification, streamlines detection by eliminating gel electrophoresis or ELISA, enhancing efficiency and minimizing contamination risks.
Size: 200 Reactions
| Components | Shipping Condition | Unit Size |
| Cell Lysis Buffer | -20℃ | 200 Reactions |
| RNase inhibitor | ||
| Telomerase positive cells Pellet | ||
| 2×TRAP qPCR Reaction Buffer | ||
| TRAP qPCR Reaction Enzyme | ||
| TRAP Primer&Control MIX | ||
| TSR8 | ||
| TRAP qPCR Reaction Replenisher |
| Range | 4x10-1-4x105 TPG Units, R2=0.980, 90%≤E≤110% |
| Accuracy | The 95% confidence interval of recovery rate is between 75% and 125% |
| LOQ | 2x10-1 TPG Units (SHENTEK-96S and ABI 7500) |
| LOD | 8x10-2 TPG Units |
| Precision | The upper limit of 95% confidence interval of RSD is within 30% |
| Instrument suitability not limited to ABI7500 and LightCycler480II |
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