Residual HPV18 E6/E7 DNA Size Analysis Kit is used to quantitate the residual HPV18 E6/E7 DNA fragments derived from HeLa cells.
SHENTEK® Residual HPV18 E6/E7 DNA Size Analysis Kit is used to quantitate residual HPV18 E6/E7 DNA of different fragment sizes of HeLa cell origin at various stages of biopharmaceutical products, from in-process samples to final products. This kit utilizes real-time PCR technique to perform rapid and specific quantitation of residual HPV18 E6/E7 DNA fragments (FAM) in samples. It is designed to amplify four different fragments (E6:100bp and 288bp; E7:110bp and 240bp) for the accurate determination of their size distribution. For extraction information, please refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191).
| Components | Shipping Condition | Unit Size | Detection Method |
| HPV18 E6/E7 DNA Control | -20℃ | 4×100 Reactions | Probe-based qPCR |
| qPCR Reaction Buffer | -20℃, protect from light | ||
| E6-100 primer&probe MIX | |||
| E6-288 prime&probe MIX | |||
| IPC MIX | |||
| DNA Dilution Buffer (DDB) | -20℃ | ||
| E7-110 primer&prob MIX | -20℃, protect from light | ||
| E7-240 primer&prob MIX | |||
| qPCR Reaction Buffer | |||
| DNA Dilution Buffer (DDB) | -20℃ |
| Range | E6 100 bp, E6 288 bp, E7 110 bp, E7 240 bp: 1x102-1x107copies /μL,R2 ≥0.990 |
| Accuracy | Recovery rate= 92.5%-108% |
| LOQ | 1x102 copies /μL |
| Precision | 4.1%-17.9% (CV≤40%) |
| Robustness | Batch to Batch consistency (CV≤30%) |
SHENTEK® Residual Host Cell DNA Sample Preparation Kit
Email: info@shentekbio.com
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