Residual HEK293 DNA Size Analysis Kit is used to quantitate HEK293 DNA residues of different fragment sizes for various stages of biopharmaceutical products, from in-process samples to final products.
The SHENTEK® Residual HEK293 DNA Size Analysis kit utilizes real-time PCR technology to perform rapid and specific quantitation of residual HEK293 DNA fragments in samples. It is designed to amplify four different fragments (75bp, 122bp, 244bp, 562bp) for the accurate determination of their size distribution with the LOD level of femtograms. For extraction information, please refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191).
| Components | Shipping Condition | Unit Size | Detection Method |
| qPCR Reaction Buffer | -20℃, protect from light | 4x100 Reactions | Probe-based qPCR |
| HEK293 Primer&Probe MIX-84 | |||
| HEK293 Primer&Probe MIX-142 | |||
| HEK293 Primer&Probe MIX-204 | |||
| HEK293 Primer&Probe MIX-504 | |||
| IPC MIX | |||
| DNA Dilution Buffer (DDB) | -20℃ | ||
| Vero DNA Control |
| Range | 3x10-2-3x102 pg/μL, R2 ≥0.990, E=83.3%-110% | ||
| Accuracy | Recovery = 76.0%-121.1%, CV<40% | ||
| LOQ | 0.03 pg/μL | ||
| Precision | 4.0%-16.2% (≤ 40%) | ||
| Robustness | Freeze-thaw stability over 5 cycles | ||
| Instrument suitability not limited to Thermo, Biorad, SHENTEK qPCR equipments | |||
SHENTEK® Residual Host Cell DNA Sample Preparation Kit
SHENTEK® Residual HEK293 DNA Quantitation Kit (3G)
SHENTEK® HEK293 HCP ELISA Kit (One-step ELISA)
info@shentekbio.com 
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