SHENTEK® Residual HEK293 DNA Quantitation Kit is used to quantitate residual HEK293 host cell DNA in different stages of biopharmaceutical products, from in-process samples to final products. This kit uses duplex real-time PCR technology to performs rapid, specific, and reliable quantitation assay at the fg level. IPC (Internal Positive Control) is included in the HEK293 Primer&Probe MIX to evaluate the performance of each PCR reaction. For extraction information, please refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191).
| Components | Shipping Condition | Unit Size | Detection Method |
| HEK293 DNA Control | -20℃ | 100 Reactions | Probe-based qPCR |
| qPCR Master MIX | -20℃, protect from light | ||
| DNA Dilution Buffer (DDB) | -20℃ | ||
| HEK293 Primer&Probe MIX (Incl IPC) | -20℃, protect from light |
| Range | 3x10-2-3x102 pg/μL, R2=0.99984, E=98.5% |
| Accuracy | Recovery = 82.1%-104.0%, CV<30% |
| LOQ | 0.03 pg/μL |
| Precision | 8.9%-15.8% (<30%) |
| Robustness | Freeze-thaw stability over 5 cycles |
| Instrument suitability not limited to Thermo, Biorad, SHENTEK qPCR equipment |
SHENTEK® Residual Host Cell DNA Sample Preparation Kit
SHENTEK® Residual HEK293 DNA Size Analysis Kit
SHENTEK® HEK293 HCP ELISA Kit (One-step ELISA)
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