Residual CHO DNA Size Analysis Kit (2G)

Name: Residual CHO DNA Size Analysis Kit (2G)
Brand: SHENTEKBIO
Availability: InStock

Product Number: 1103170-2

Residual CHO DNA Size Analysis Kit is used to quantitate CHO DNA residues of different fragment sizes in different stages of biopharmaceutical products, from in-process samples to final products.

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Overview - Residual CHO DNA Size Analysis Kit (2G)

SHENTEK^® Residual CHO DNA Size Analysis Kit (2G) is used to quantitate CHO DNA residues of different fragment sizes in different stages of biopharmaceutical products, from in-process samples to final products. This kit utilizes fluorescent quantitative PCR technique (FAM) to perform rapid and specific quantitation of residual CHO DNA fragments in samples. The assay is designed to amplify four different fragments (95bp, 110bp, 215bp, 523bp) for the accurate size distribution, and the detection limit reaches femtograms (fg) level. For extraction information, please refer to the SHENTEK^® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191).

Introduction - Residual CHO DNA Size Analysis Kit (2G)

Components	Shipping Condition	Unit Size	Detection Method
qPCR Reaction Buffer	-20℃, protect from light	4×100 Reactions	Probe-based qPCR
CHO Primer&Probe MIX-95
CHO Primer&Probe MIX-110
CHO Primer&Probe MIX-215
CHO Primer&Probe MIX-523
IPC MIX
CHO DNA Control	-20℃
DNA Dilution Buffer (DDB)	-20℃

Specification - Residual CHO DNA Size Analysis Kit (2G)

Range	95bp, 110bp, 215bp, 523bp: 3x10^-2-3x10² pg/μL, R² ≥0.990, E= 83.3%-110%
Accuracy	Recovery rate = 89.3%-119.2%
LOQ	0.03 pg/μL
Precision	11.9%-29.5% (CV≤40%)
Robustness	Batch-to-batch precision (CV≤20%)