Kanamycin ELISA Kit

Name: Kanamycin ELISA Kit
Brand: SHENTEKBIO
Availability: InStock

Product Number: 1401402

This kits intended for quantitative determination of kanamycin residues in plasmid DNA, that is used in the development of cell and gene therapies.

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Overview - SHENTEK^® Kanamycin ELISA Kit

The SHENTEK^®Kanamycin ELISA Kit utilizes an "indirect-competitive" enzyme immunoassay. The microplate wells are coated with kanamycin antigen, that competes with Kanamycin in a sample for anti-kanamycin monoclonal antibody. An enzyme-tagged secondary antibody targets the primary monoclonal antibody that is complexed with the kanamycin coated on the plate wells. After an addition of TMB substrate, the resulting color intensity at 450nm has an inverse relationship with the Kanamycin residue concentration in the sample. The total amount of kanamycin in the sample is obtained by comparison with a standard curve, and adjusted by the sample dilution factor.

For the detection of residual kanamycin in other biologics samples, sample suitability test is recommended to assess the effects of matrix interference.

Introduction -SHENTEK^®Kanamycin ELISA Kit

Components	Shipping Condition	Unit Size	Detection Method
Kanamycin Standard	2-8℃, protect from light	96 Tests	Sandwich ELISA
Kanamycin Standard Reconstitution Diluent	2-8℃
Kanamycin Standard Solution Diluent	2-8℃
100×Kanamycin Primary Antibody	2-8℃
100×Enzyme-conjugated Secondary Antibody	2-8℃, protect from light
Kanamycin-coated Plate	2-8℃, protect from light
10×Wash Buffer	2-8℃
Chromogenic Substrate for Kanamycin ELISA	2-8℃, protect from light
Stop Solution	2-8℃
Sealing Film	2-8℃

Specification - SHENTEK^® Kanamycin ELISA Kit

Linearity & Range	0.5-32 ng/mL, R²>0.990
Accuracy	Recovery Rate= 80.8%-112.5%
LLOQ	0.5 ng/mL
ULOQ	16 ng/mL
Repeatability	5.3%-9.1% (CV≤20%)
Specificity	No cross-reactivity with tetracycline, streptomycin sulfate, ampicillin, and chloramphenicol
Robustness	Incubate at 22℃ to 30℃, CV≤ 20%
Robustness	Instrument suitability not limited to Thermo Multiskan FC, Bio-Tek Synergy2