Human IL-6 ELISA Kit

Overview - SHENTEK^® Human IL-6 ELISA Kit

This kit is based on the solid-phase enzyme-linked immunosorbent assay (ELISA) with a

double-antibody sandwich technique to determine the amount of hIL-6 in biological

products. A hIL-6-specific capture antibody was pre-coated into each well of microtiter

strips. Both Calibration Standards and test samples were simultaneously added to the

microtiter strips, and followed by incubation and washing. The biotinylated detection

antibody was added to the microtiter strips to bind the epitope of hIL-6, thus forming a

sandwich structure, which further reacted with streptavidin-labeled HRP (SA-HRP). TMB

substrate was added into reaction, catalyzed by enzymatic hydrolysis to produce a blue

colored product (maximum absorption peak at 655 nm). Finally, a stop solution was added

to terminate the enzymatic reaction, resulting in a yellow colored product (maximum

absorption peak at 450 nm). The absorbance values at 450 nm wavelength were positively

correlated with the hIL-6 concentration in the Calibration Standards and the samples. The

concentration of hIL-6 in the sample can be calculated using a dose-response curve.

Introduction-SHENTEK^®Human IL-6 ELISA Kit

Specification - SHENTEK^® Human IL-6 ELISA Kit

Related Products

SHENTEK^® Residual Host Cell DNA Sample Preparation Kit