MAT Kit

Name: MAT Kit
Brand: SHENTEKBIO
Availability: InStock

Product Number: 1502100

With the European Pharmacopoeia phasing out the Rabbit Pyrogen Test (RPT), the Monocyte Activation Test (MAT) has emerged as a state-of-the-art, animal-free in vitro assay, utilizing human primary monocytes or monocyte cell lines to detect pyrogens with high physiological relevance.

MAT enables the detection of both endotoxins and non-endotoxin pyrogens (NEPs), providing broader pyrogen coverage and improved risk control, supporting alignment with the global 3R principles (Replacement, Reduction, and Refinement) and evolving regulatory expectations for modern biopharmaceutical quality control.

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Overview - PyroSup^® MAT Kit

The Monocyte Activation Test (MAT) is an in vitro assay designed to detect pyrogenic substances in pharmaceutical, biological products and medical apparatus by measuring the immune response of human monocytes.

Monocytes, as key components of the human innate immune system, recognize pyrogens through Toll-like receptors (TLRs) and respond by releasing proinflammatory cytokines, such as interleukin-6 (IL-6). The concentration of IL-6 secreted into the culture supernatant reflects the degree of pyrogenic stimulation.

The PyroSup® MAT Kit utilizes cryopreserved human monocytic cells (MAT cells) and a human IL-6 ELISA assay to quantitatively determine the presence of pyrogens in test samples. This method enables detection of both endotoxin and non-endotoxin pyrogens (NEPs), providing a physiologically relevant alternative to traditional rabbit pyrogen test (PRT). Prior to routine testing, the assay should be validated for each sample to exclude potential interference and confirm assay sensitivity for both endotoxin and non-endotoxin pyrogens.

Introduction - PyroSup^® MAT Kit

Components	Shipping Condition	Unit Size	Detection Method
Culture medium	2-8℃	96 Tests	Monocyte Activation
96-well plate	2-8℃
Water for BET	2-8℃
Anti-hIL-6: Biotinylated Conjugate (200×)	2-8℃, protect from light
Streptavidin-HRP (100×)	2-8℃, protect from light
Diluent	2-8℃
Wash Buffer Concentrate (10×)	2-8℃
TMB Substrate	2-8℃, protect from light
Stop Solution	2-8℃
Sealing Film	2-8℃
Anti-hIL-6 Microtiter Strips	2-8℃, protect from light
Culture medium supplement	-20℃
Reference Standard Endotoxin (RSE)	-20℃
MAT cells	Liquid nitrogen

Features - PyroSup^® MAT Kit

Linearity	0.0125 - 1 EU/mL, R2 ≥ 0.980
LOQ	0.025 EU/mL
LOD	0.0125 EU/mL
Precision	CV ≤ 15%
Specificity	Detect at least two kinds of non-endotoxin pyrogen, lipoteichoic acid (LTA) and resiquimod
Robustness	Batch-to-batch Consistency
Robustness	Instrument suitability not limited to Thermo Multiskan FC and MD Spectra Max M2