The AdvSHENTEK Mycoplasma DetectInnova Cassette, designed for use with the AdvSHENTEK DetectInnova System, uses magnetic particle separation technology and real-time fluorescence PCR technology. The cassette is a closed, disposable system, which contains all necessary reagents for nucleic acid extraction, purification, amplification and detection, enabling an efficient, all-in-one workflow from sample preparation to result analysis. After sampling or simplified preparation, the user pipettes Magnetic Particles and sample combined with IC into the cassette, inserts the cassette into an AdvSHENTEK DetectInnova System, and starts a test. The entire test takes approximately 3 hours. For more information, please refer to the Operating Manual for the Mycoplasma DetectInnova System.
| Components | Shipping Condition | Unit Size | Detection Method |
| AdvSHENTEK Mycoplasma DetectInnova Cassette I | 2-8℃ | 24 Cassettes/Kit | Probe-based qPCR |
| MPDI2 Cassette* | |||
| *The cassette name on the QR code label must exactly match the test program. Scanning the QR code automatically assigns the execution program on the instrument. | |||
| AdvSHENTEK Mycoplasma DetectInnova Cassette II | |||
| MP Internal Control | |||
| MP Positive Control | |||
| Lysis Buffer | |||
| Magnetic Particles | |||
| Saline Buffer | |||
| Digestion Buffer | |||
| IC Buffer | |||
| Sample Buffer | |||
Note: Protect the cassette from light after opening. The kit components can be stored under the specified conditions for up to 12 months. Verify the expiration date on the label. |
| LOD | 10 CFU/mL |
| Limit of Blank | NCS (FAM): no amplification curves or Ct≥40 |
| Internal control, ROX: Ct<35 | |
| Specificity | No interference was showed with 4 types of sample matrix (DMEM (20% FBS + 10% DMSO), DMEM (10% FBS), ExpiCHOTM, ExpiCHOTM (10%DMSO) ) |
| Negative results were obtained from testing 18 genomic DNAs (engineered cells and bacteria) | |
| Strain Specificity | Bioinformatics analysis shows the system can detect nearly 200 species genomic DNA |
| Positive results was showed using eleven control strains (Mycoplasma orale, Mycoplasma pneumoniae, Mycoplasma hominis, Acholeplasma laidlawii, Mycoplasma salivarium, Mycoplasma fermentans, Spiroplasma citri, Mycoplasma hyorhinis, Mycoplasma arginine, Mycoplasma synoviae, and Mycoplasma gallisepticum) | |
| Robustness | Detect 10 CFU/mL of Mycoplasma species (orale, hyorhinis, pneumoniae, fermentans) in high-density CHO-S cell substrate (10⁷ cells/mL) with >95% detection rates. |
| Sample volume: 0.2ml-1ml | |
| Repeatability | Instrument Precision (CV ≤ 15%) |
SHENTEK® AdvSHENTEK DetectInnova System
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